Oral History Interview: Mary Carmichael

This interview is one of a series conducted with former employees of the Huntington Owens-Illinois, Inc. glass bottle factory. Mrs. Mary Carmichael, born January 12, 1920, began working at the Owens plant in the corrugated department in 1943. She was transferred to the selecting department when automation displaced her from her job in corrugated. Later, Mrs. Carmichael was promoted and worked as an inspector in the selecting department. In this interview, Mrs. Carmichael discusses the details of the jobs she performed, union activities, strikes, automation, and company-sponsored activities. Furthermore, she talks about the plant production during WWII and the issue of job segregation. Finally, Mrs. Carmichael talks about the physical problems she continues to have because of the strenuous work she performed at the glass factory.https://mds.marshall.edu/oral_history/1399/thumbnail.jp

Shaping (reflexive) professional identities across an undergraduate degree programme : A longitudinal case study

In our complex and incongruous professional worlds, where there is no blueprint for dealing with unpredictable people and events, it is imperative that individuals develop reflexive approaches to professional identity building. Notwithstanding the importance of disciplinary knowledge and skills, higher education has a crucial role to play in guiding students to examine and mediate self in relation to context for effective decision-making and action. This paper reports on a small-scale longitudinal project that investigated the ways in which 10 undergraduate students over the course of a three-year Radiation Therapy degree shaped their professional identities. Theories of reflexivity and methods of discourse analysis are utilised to understand the ways in which individuals accounted for their professional identity projects at university. The findings suggest that, across time, the participants negotiated professional ‘becoming’ through four distinct kinds of reflexive modalities. These findings have implications for teaching strategies and curriculum design in undergraduate programmes

Alien Registration- Carmichael, Mary J. (Limestone, Aroostook County)

https://digitalmaine.com/alien_docs/35231/thumbnail.jp

Alien Registration- Carmichael, Mary J. (Limestone, Aroostook County)

https://digitalmaine.com/alien_docs/35231/thumbnail.jp

Mn(II)-oxidizing Bacteria are Abundant and Environmentally Relevant Members of Ferromanganese Deposits in Caves of the Upper Tennessee River Basin

The upper Tennessee River Basin contains the highest density of our nation's caves; yet, little is known regarding speleogenesis or Fe and Mn biomineralization in these predominantly epigenic systems. Mn:Fe ratios of Mn and Fe oxide-rich biofilms, coatings, and mineral crusts that were abundant in several different caves ranged from ca. 0.1 to 1.0 as measured using ICP-OES. At sites where the Mn:Fe ratio approached 1.0 this represented an order of magnitude increase above the bulk bedrock ratio, suggesting that biomineralization processes play an important role in the formation of these cave ferromanganese deposits. Estimates of total bacterial SSU rRNA genes in ferromanganese biofilms, coatings, and crusts measured approximately 7×107–9×109 cells/g wet weight sample. A SSU-rRNA based molecular survey of biofilm material revealed that 21% of the 34 recovered dominant (non-singleton) OTUs were closely related to known metal-oxidizing bacteria or clones isolated from oxidized metal deposits. Several different isolates that promote the oxidation of Mn(II) compounds were obtained in this study, some from high dilutions (10–8–10–10) of deposit material. In contrast to studies of caves in other regions, SSU rRNA sequences of Mn-oxidizing bacterial isolates in this study most closely matched those of Pseudomonas, Leptothrix, Flavobacterium, and Janthinobacterium. Combined data from geochemical analyses, molecular surveys, and culture-based experiments suggest that a unique consortia of Mn(II)-oxidizing bacteria are abundant and promoting biomineralization processes within the caves of the upper Tennessee River Basin

Sustained Anthropogenic Impact in Carter Saltpeter Cave, Carter County, Tennessee and the Potential Effects On Manganese Cycling

Anthropogenic impact is a pervasive problem in heavily trafficked cave systems and fecal contamination is equally problematic in many cave and karst waters worldwide. Carter Saltpeter Cave in Carter County, Tennessee exhibits Mn(III/IV) oxide coatings associated with groundwater seeps, as well as manganese oxide growth on litter. Culturing results revealed that Mn(III/IV) oxide production on litter was associated with Mn(II)-oxidizing fungi. Immediately prior to this study, a massive Mn(II)-oxidizing biofilm bloomed at a cave seep. During the course of this study from 2009–2011, the seep exhibited a dramatic visual reduction in Mn(III/IV) oxide production, which was hypothesized to correlate with a decrease in fecal nutrient input. Molecular methods (16S rRNA gene sequencing) confirmed the presence of Bacteroides-Prevotella human fecal indicators in this seep, and most probable number assays and ion chromatography of the associated seep water confirmed nutrient loading at the site. Further, phylogenetic analysis from clone sequences suggested a strong initial human-specific fecal signature (50% of the sequences clustering with human feces sequences) in July 2009, and a weaker human signature (20% clustering) by June 2011. Most Probable Number (MPN) analyses of heterotrophic bacteria at this site suggested that Mn(II) oxidation was correlated with heterotrophic activity, due to point source exogenous nutrient loading

Robust generation of entanglement in Bose-Einstein condensates by collective atomic recoil

We address the dynamics induced by collective atomic recoil in a Bose-Einstein condensate in presence of radiation losses and atomic decoherence. In particular, we focus on the linear regime of the lasing mechanism, and analyze the effects of losses and decoherence on the generation of entanglement. The dynamics is that of three bosons, two atomic modes interacting with a single-mode radiation field, coupled with a bath of oscillators. The resulting three-mode dissipative Master equation is solved analytically in terms of the Wigner function. We examine in details the two complementary limits of {\em high-Q cavity} and {\em bad-cavity}, the latter corresponding to the so-called superradiant regime, both in the quasi-classical and quantum regimes. We found that three-mode entanglement as well as two-mode atom-atom and atom-radiation entanglement is generally robust against losses and decoherence,thus making the present system a good candidate for the experimental observation of entanglement in condensate systems. In particular, steady-state entanglement may be obtained both between atoms with opposite momenta and between atoms and photons

Mn(II)-Oxidizing Bacteria Are Abundant And Environmentally Relevant Members Of Ferromanganese Deposits In Caves Of The Upper Tennessee River Basin

The upper Tennessee River Basin contains the highest density of our nation’s caves; yet, little is known regarding speleogenesis or Fe and Mn biomineralization in these predominantly epigenic systems. Mn:Fe ratios of Mn and Fe oxide-rich bio?lms, coatings, and mineral crusts that were abundant in several different caves ranged from ca. 0.1 to 1.0 as measured using ICP-OES. At sites where the Mn:Fe ratio approached 1.0 this represented an order of magnitude increase above the bulk bedrock ratio, suggesting that biomineralization processes play an important role in the formation of these cave ferromanganese deposits. Estimates of total bacterial SSU rRNA genes in ferromanganese bio?lms, coatings, and crusts measured approximately 7×107–9×109 cells/g wet weight sample. A SSU-rRNA based molecular survey of bio?lm material revealed that 21% of the 34 recovered dominant (non-singleton) OTUs were closely related to known metal-oxidizing bacteria or clones isolated from oxidized metal deposits. Several different isolates that promote the oxidation of Mn(II) compounds were obtained in this study, some from high dilutions (10–8–10–10) of deposit material. In contrast to studies of caves in other regions, SSU rRNA sequences of Mn-oxidizing bacterial isolates in this study most closely matched those of Pseudomonas, Leptothrix, Flavobacterium, and Janthinobacterium. Combined data from geochemical analyses, molecular surveys, and culture-based experiments suggest that a unique consortia of Mn(II)-oxidizing bacteria are abundant and promoting biomineralization processes within the caves of the upper Tennessee River Basin

Region-specific and activity-dependent regulation of SVZ neurogenesis and recovery after stroke.

Stroke is the leading cause of adult disability. Neurogenesis after stroke is associated with repair; however, the mechanisms regulating poststroke neurogenesis and its functional effect remain unclear. Here, we investigate multiple mechanistic routes of induced neurogenesis in the poststroke brain, using both a forelimb overuse manipulation that models a clinical neurorehabilitation paradigm, as well as local manipulation of cellular activity in the peri-infarct cortex. Increased activity in the forelimb peri-infarct cortex via either modulation drives increased subventricular zone (SVZ) progenitor proliferation, migration, and neuronal maturation in peri-infarct cortex. This effect is sensitive to competition from neighboring brain regions. By using orthogonal tract tracing and rabies virus approaches in transgenic SVZ-lineage-tracing mice, SVZ-derived neurons synaptically integrate into the peri-infarct cortex; these effects are enhanced with forelimb overuse. Synaptic transmission from these newborn SVZ-derived neurons is critical for spontaneous recovery after stroke, as tetanus neurotoxin silencing specifically of the SVZ-derived neurons disrupts the formation of these synaptic connections and hinders functional recovery after stroke. SVZ-derived neurogenesis after stroke is activity-dependent, region-specific, and sensitive to modulation, and the synaptic connections formed by these newborn cells are functionally critical for poststroke recovery

Radiation damage to nucleoprotein complexes in macromolecular crystallography

Significant progress has been made in macromolecular crystallography over recent years in both the understanding and mitigation of X-ray induced radiation damage when collecting diffraction data from crystalline proteins. In contrast, despite the large field that is productively engaged in the study of radiation chemistry of nucleic acids, particularly of DNA, there are currently very few X-ray crystallographic studies on radiation damage mechanisms in nucleic acids. Quantitative comparison of damage to protein and DNA crystals separately is challenging, but many of the issues are circumvented by studying pre-formed biological nucleoprotein complexes where direct comparison of each component can be made under the same controlled conditions. Here a model protein-DNA complex C.Esp1396I is employed to investigate specific damage mechanisms for protein and DNA in a biologically relevant complex over a large dose range (2.07-44.63 MGy). In order to allow a quantitative analysis of radiation damage sites from a complex series of macromolecular diffraction data, a computational method has been developed that is generally applicable to the field. Typical specific damage was observed for both the protein on particular amino acids and for the DNA on, for example, the cleavage of base-sugar N1-C and sugar-phosphate C-O bonds. Strikingly the DNA component was determined to be far more resistant to specific damage than the protein for the investigated dose range. At low doses the protein was observed to be susceptible to radiation damage while the DNA was far more resistant, damage only being observed at significantly higher doses